| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha, Ago2 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
promotes glycolysis and growth/proliferation of gastric cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a ceRNA sponging miR-515-5p to increase HIF-1alpha expression; HIF-1alpha binds the circ-MAT2B promoter and activates circ-MAT2B transcription, forming a positive feedback loop |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Western Blot; CCK8; Colony Formation Assay; EdU Staining; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis |
| Clinical significance: |
High circ-MAT2B expression is associated with aggressive clinicopathological features, has diagnostic value in plasma (AUC 0.8875), and predicts unfavorable prognosis (shorter OS and DFS). |
| Description: |
circ-MAT2B is up-regulated in gastric cancer tissues and plasma and promotes GC cell proliferation and glycolysis. It functions mainly in the cytoplasm as a ceRNA that sponges miR-515-5p to increase HIF-1alpha, and HIF-1alpha transcriptionally activates circ-MAT2B to form a positive feedback loop. High circ-MAT2B is associated with aggressive clinicopathological features and poor survival, supporting diagnostic and prognostic value. |
| Confidence score: |
0.8246 |