circRNA basic information
circBase ID: hsa_circ_0011385
Name: hsa_circ_EIF3I
Synonym: -
Host Gene: EIF3I
Genomic location(hg19): chr1:32691771-32692131:+
Genomic location(hg38): chr1:32226170-32226530:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; matching adjacent normal tissues; tumor tissues
Cell lines:

293T; HUVECs; H8; HeLa; SiHa
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PRDX6, EIF3I, MMP2, MMP9, Ago2, Ki-67, PCNA
Associated microRNA: miR-149-5p
Biological function: Promotes cervical cancer cell proliferation, colony formation, cell cycle progression, migration, invasion, angiogenesis, and in vivo tumor growth; inhibits apoptosis and cell cycle arrest.
Molecular mechanism: hsa_circ_0011385 functions as a ceRNA/miRNA sponge for miR-149-5p, thereby increasing PRDX6 expression and promoting angiogenesis and malignant behaviors in cervical cancer cells.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); angiogenesis (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High hsa_circ_0011385 expression was associated with histologic grade and FIGO stage, predicted shorter overall survival, and hsa_circ_0011385 was proposed as a prognostic biomarker and therapeutic target for cervical cancer.
Description:

hsa_circ_0011385 is up-regulated in cervical cancer tissues and cell lines and high expression is associated with poorer overall survival. Functionally, it promotes CC cell proliferation, migration, invasion, angiogenesis, and xenograft tumor growth while suppressing apoptosis. Mechanistically, cytoplasmic hsa_circ_0011385 sponges miR-149-5p to increase PRDX6 expression, forming a hsa_circ_0011385/miR-149-5p/PRDX6 regulatory axis.
Confidence score:

0.849
Other information
Title:

Circular RNA hsa_circ_0011385 contributes to cervical cancer progression through sequestering miR-149-5p and increasing PRDX6 expression.

Journal: Reproductive biology
Published: 2022
PubMed ID: 35240453
Study type:

combined biological and clinical study
Data availability: GSE102686; GSE113696; doi:10.1016/j.repbio.2022.100619
Code availability: -