| Expression pattern: |
UP |
| Associated gene: |
NOTCH2, Ago2 |
| Associated microRNA: |
miR-375, miR-1231 |
| Biological function: |
promotes bladder cancer cell proliferation, colony formation, invasion and metastasis; knockdown inhibits tumor growth and lung metastasis |
| Molecular mechanism: |
circKIF4A acts as a ceRNA/miRNA sponge for miR-375 and miR-1231, relieving repression of NOTCH2 and promoting PI3K-AKT signaling |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circKIF4A (hsa_circ_0007255) is up-regulated in bladder cancer tissues and cell lines and promotes proliferation, invasion and metastasis. It mainly localizes to the cytoplasm and functions as a ceRNA sponging miR-375 and miR-1231 to upregulate NOTCH2, with downstream involvement of PI3K/AKT signaling. |
| Confidence score: |
0.7302 |