| Expression pattern: |
UP |
| Associated gene: |
PTP4A1, ERK1/2, MMP2, MMP9, ZEB2, E-cadherin |
| Associated microRNA: |
miR-330-5p, miR-339-5p, miR-595, miR-629-3p, miR-653-5p |
| Biological function: |
promotes cervical cancer cell proliferation, migration, and invasion; promotes tumor growth in vivo; involved in EMT |
| Molecular mechanism: |
acts as a miRNA sponge for miR-629-3p to regulate PTP4A1 and the ERK1/2 pathway, affecting downstream MMP2/MMP9 and EMT markers |
| Biological pathway or process: |
ERK (promotes); migration (promotes); invasion (promotes); proliferation (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circNRIP1 expression was correlated with lymphovascular space invasion (LVSI) and proposed as a potential prognostic biomarker and therapeutic target in CC patients |
| Description: |
circNRIP1 (hsa_circ_0004771), derived from NRIP1, is up-regulated in cervical cancer tissues and promotes proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it is mainly cytoplasmic and acts as a ceRNA by sponging miR-629-3p, thereby increasing PTP4A1 and activating ERK1/2 signaling with downstream changes including MMP2/MMP9 and EMT-related markers. |
| Confidence score: |
0.8124 |