| Expression pattern: |
DN |
| Associated gene: |
WTAP, EIF3C, METTL3, METTL14, YTHDF1, FOXO4, eIF4A3 |
| Associated microRNA: |
- |
| Biological function: |
inhibits migration and invasion; restrains metastasis |
| Molecular mechanism: |
Forms a circPDE5A-WTAP complex to block WTAP-dependent m6A methylation of EIF3C mRNA, reducing EIF3C translation (YTHDF1-dependent) and inactivating the MAPK pathway. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); m6A modification (inhibits); MAPK (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; MeRIP / MeRIP-seq; Luciferase Reporter Assay; ChIP / ChIP-seq; Transwell Assay; CCK8; In Vivo Animal Model; H&E Staining; Western Blot; IF (Immunofluorescence); Bioinformatics Analysis; CLIP |
| Clinical significance: |
downregulated in PCa tissues compared to adjacent normal tissues; negatively correlated with gleason score; could be a potential diagnostic biomarker and an effective therapeutic target for PCa treatment |
| Description: |
circPDE5A is down-regulated in prostate cancer and functions as a metastasis suppressor. It binds WTAP and inhibits WTAP-dependent m6A methylation on EIF3C mRNA, reducing YTHDF1-associated translational output of EIF3C and thereby inactivating MAPK signaling to restrain migration/invasion and metastasis. |
| Confidence score: |
0.8304 |