circRNA basic information
circBase ID: hsa_circ_0089762
Name: hsa_circ_COX2
Synonym: -
Host Gene: COX2
Genomic location(hg19): chrM:7586-7982:-
Genomic location(hg38): chrM:7585-7981:-
Subcellular localization: mitochondria
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0003864
MONDO name: chronic lymphocytic leukemia/small lymphocytic lymphoma
Disease details: chronic lymphocytic leukemia
Disease DO ID:
6354
Disease MeSH ID:
-
Disease NCIt ID:
C27911
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma; plasma exosomes

Cell lines:

MEC-1; JVM-3; HEK293T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: AGO2, TP53
Associated microRNA: -
Biological function: Promotes CLL cell proliferation/viability, inhibits apoptosis, and modulates mitochondrial function; its silencing enhances anti-tumor drug effects.
Molecular mechanism: Does not act as a ceRNA (fails to bind AGO2); influences mitochondrial membrane potential and ATP production; mitochondrial-targeting compounds reduce mc-COX2 biogenesis and synergize with mc-COX2 knockdown.
Biological pathway or process:

mitochondrial function (other); proliferation (promotes); apoptosis (inhibits); drug resistance (inhibits)

Detected method:
Q
B
H
M
Validation methods:

Microarray; RT-qPCR; FISH / smFISH; Northern Blot; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Western Blot; Survival Analysis; Clinical Sample Validation; Bioinformatics Analysis

Clinical significance:

High plasma/exosomal mc-COX2 predicts worse overall survival in CLL patients and is proposed as a prognostic biomarker/liquid biopsy target.

Description:

mc-COX2 (hsa_circ_0089762), an mt-circRNA derived from mitochondrial COX2, is up-regulated in CLL plasma and enriched in plasma exosomes. mc-COX2 promotes CLL cell viability/proliferation, supports mitochondrial function, and inhibits apoptosis; high mc-COX2 is associated with worse overall survival. Mechanistically, mc-COX2 does not bind AGO2 and may act through non-ceRNA mechanisms; targeting mc-COX2 enhances responses to mitochondrial-related compounds and inhibitors.

Confidence score:

0.8478

Other information
Title:

Mitochondrial Genome-Derived circRNA mc-COX2 Functions as an Oncogene in Chronic Lymphocytic Leukemia.

Journal: Molecular therapy. Nucleic acids
Published: 2020
PubMed ID: 32438315
Study type:

combined biological and clinical study

Data availability: https://doi.org/10.1016/j.omtn.2020.04.017
Code availability: -