| Expression pattern: |
UP |
| Associated gene: |
FAM83D, Ago2, CCND1, CCNE1 |
| Associated microRNA: |
miR-614 |
| Biological function: |
promotes cell proliferation; promotes cell cycle progression; promotes tumor growth |
| Molecular mechanism: |
circFOXM1 acts as a ceRNA/miRNA sponge for miR-614, relieving miR-614-mediated repression of FAM83D and increasing FAM83D expression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; RNA-seq; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circFOXM1 was positively correlated with advanced clinical stage and poor prognosis of NSCLC patients. |
| Description: |
circFOXM1 is up-regulated in NSCLC and promotes proliferation/cell-cycle progression and xenograft tumor growth. It mainly localizes in the cytoplasm and functions as a ceRNA by sponging miR-614, thereby de-repressing FAM83D and increasing downstream cell-cycle proteins (e.g., CCND1, CCNE1). High circFOXM1 expression is associated with advanced stage and poor overall survival. |
| Confidence score: |
0.8662 |