circRNA basic information
circBase ID: hsa_circ_0000615
Name: hsa_circ_ZNF609
Synonym: circZNF609
Host Gene: ZNF609
Genomic location(hg19): chr15:64791491-64792365:+
Genomic location(hg38): chr15:64499292-64500166:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; peri-tumor tissues
Cell lines:

HepG2; Huh-7; HCCLM3; MHCC-97H; THLE-3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: GLI2, Ago2, Hedgehog pathway
Associated microRNA: miR-15a-5p, miR-15b-5p
Biological function: promotes cell proliferation, metastasis and stemness; inhibits apoptosis; promotes in vivo tumorigenesis
Molecular mechanism: ceRNA mechanism: circZNF609 sponges miR-15a-5p/miR-15b-5p to upregulate GLI2 and activate Hedgehog pathway
Biological pathway or process:

Hedgehog (promotes); proliferation (promotes); metastasis (promotes); stemness (promotes); apoptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; TUNEL; Western Blot; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; Microarray
Clinical significance:

-
Description:

circZNF609 is up-regulated in HCC tissues and cell lines and mainly localizes to the cytoplasm. It promotes proliferation, migration/invasion, EMT and stemness while inhibiting apoptosis by sponging miR-15a-5p/miR-15b-5p, thereby increasing GLI2 expression and activating Hedgehog signaling; knockdown suppresses HCC xenograft growth.
Confidence score:

0.852
Other information
Title:

CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions.

Journal: Cell death & disease
Published: 2020
PubMed ID: 32398664
Study type:

combined biological and clinical study
Data availability: Supplementary Information accompanies this paper at (10.1038/s41419-020-2441-0).
Code availability: -