| Expression pattern: |
UP |
| Associated gene: |
SOX9, SLC30A7 |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
Promotes CRC cell colony formation, proliferation, viability, migration, invasion, and xenograft tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0013339 functions as a ceRNA for miR-136-5p to elevate SOX9 expression and regulate CRC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; CCK8; Western Blot; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Provides a new biomarker for the treatment and prognosis of CRC patients. |
| Description: |
circ_0013339 is up-regulated in colorectal cancer tissues and cells and acts as an oncogenic circRNA. It promotes CRC cell proliferation, viability, migration, invasion, and xenograft tumor growth while suppressing apoptosis, mainly through a ceRNA mechanism involving the miR-136-5p/SOX9 axis. The study proposes circ_0013339 as a potential biomarker and therapeutic target for CRC. |
| Confidence score: |
0.8175 |