| Expression pattern: |
UP |
| Associated gene: |
OLFM4, STAT3, p-STAT3 |
| Associated microRNA: |
miR-502-3p |
| Biological function: |
Promotes gastric cancer cell proliferation, migration, invasion and in vivo tumor growth; inhibits apoptosis; functions as an oncogene in gastric cancer. |
| Molecular mechanism: |
circ-RPL15 acts as a competitive endogenous RNA by sponging miR-502-3p, thereby regulating OLFM4 and activating the STAT3 pathway. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (other); JAK/STAT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; FISH / smFISH; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Overexpressed circ-RPL15 predicts poor overall survival and is associated with larger tumor sizes, higher TNM stages and earlier distant metastasis in gastric cancer patients. |
| Description: |
circ-RPL15 is up-regulated in gastric cancer tissues and cell lines, and high expression predicts poorer overall survival and more aggressive clinicopathological features. Functionally, circ-RPL15 promotes gastric cancer proliferation, migration, invasion and xenograft tumor growth while inhibiting apoptosis. Mechanistically, it acts as a ceRNA for miR-502-3p to regulate OLFM4 and activate the STAT3 pathway. |
| Confidence score: |
0.7369 |