| Expression pattern: |
DN |
| Associated gene: |
MYPT1, p-MLC2, URH49 |
| Associated microRNA: |
miR-141-3p |
| Biological function: |
suppresses migration, invasion, amoeboid migration, and metastasis |
| Molecular mechanism: |
circSOBP sponges miR-141-3p to regulate MYPT1 and p-MLC2 (MYPT1/p-MLC2 axis), thereby controlling amoeboid migration |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Luciferase Reporter Assay; Transfection; Transwell Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circSOBP level was correlated with the grade group of PCa; ROC AUC=0.763 for distinguishing PCa and ANP tissues |
| Description: |
circSOBP (hsa_circ_0001633), derived from SOBP, is down-regulated in prostate cancer and suppresses migration, invasion, and in vivo metastasis. It mainly localizes to the cytoplasm and acts as a miR-141-3p sponge to upregulate MYPT1, thereby modulating the MYPT1/p-MLC2 axis to inhibit amoeboid migration. |
| Confidence score: |
0.8426 |