circRNA basic information
circBase ID: -
Name: hsa_circ_MYLK
Synonym: -
Host Gene: MYLK
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RCC tissues; matched adjacent normal renal tissues
Cell lines:

HK-2 / ACHN / 786-O / Caki-2 / 293-T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: VEGFC
Associated microRNA: miR-513a-5p
Biological function: promotes RCC growth, proliferation, migration, invasion, EMT and metastasis
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-513a-5p to increase VEGFC expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation
Clinical significance:

Increased circMYLK expression led to a larger tumour size, distant metastasis and poor prognosis of RCC patients.
Description:

circMYLK is up-regulated in renal cell carcinoma and is associated with larger tumor size, distant metastasis, and poorer overall survival. Functionally, circMYLK promotes RCC proliferation, migration/invasion, EMT, and metastasis. Mechanistically, it localizes mainly in the cytoplasm and acts as a ceRNA that sponges miR-513a-5p to increase VEGFC expression.
Confidence score:

0.8037
Other information
Title:

Circular RNA MYLK promotes tumour growth and metastasis via modulating miR-513a-5p/VEGFC signalling in renal cell carcinoma.

Journal: Journal of cellular and molecular medicine
Published: 2020
PubMed ID: 32342645
Study type:

combined biological and clinical study
Data availability: The data set(s) supporting the findings of this study are included within the article.
Code availability: -