circRNA basic information
circBase ID: hsa_circ_0000247
Name: hsa_circ_MCU
Synonym: -
Host Gene: MCU
Genomic location(hg19): chr10:74474868-74475660:+
Genomic location(hg38): chr10:72715110-72715902:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent normal breast tissues
Cell lines:

MDA-MB-231; MDA-MB-468; MCF7; MCF-10A; MCF-7; SKBR3; 4175; SCP2; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AGO2, MYC, HMGA2, CCND1
Associated microRNA: let-7 family, let-7a, let-7c, let-7f
Biological function: promotes proliferation, migration, invasion and lung metastasis; affects the G1 phase cell cycle checkpoint; promotes EMT; decreases apoptosis
Molecular mechanism: acts as a ceRNA/miRNA sponge for let-7 family, reducing let-7 suppression of targets and increasing MYC, HMGA2 and CCND1 expression in an AGO2-associated manner
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); apoptosis (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; divergent primers PCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Colony Formation Assay; EdU Staining; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis
Clinical significance:

high circHMCU expression was significantly correlated with a poor prognosis in breast cancer patients; positively correlated with histological grade, lymph node metastasis, T stage and N stage
Description:

circHMCU (hsa_circ_0000247), derived from MCU, is up-regulated in breast cancer tissues and aggressive/metastatic breast cancer cell sublines and is mainly cytoplasmic. It promotes proliferation and metastasis (EMT, migration/invasion, lung metastasis) by sponging let-7 family miRNAs, thereby increasing let-7 target genes including MYC, HMGA2 and CCND1; high circHMCU is associated with poor patient prognosis.
Confidence score:

0.8824
Other information
Title:

circHMCU Promotes Proliferation and Metastasis of Breast Cancer by Sponging the let-7 Family.

Journal: Molecular therapy. Nucleic acids
Published: 2020
PubMed ID: 32330870
Study type:

combined biological and clinical study
Data availability: GSE111504
Code availability: -