| Expression pattern: |
UP |
| Associated gene: |
ELK1, Ago2 |
| Associated microRNA: |
miR197-3p |
| Biological function: |
promotes cell viability and colony formation, facilitates cell-cycle progression, inhibits apoptosis, and inhibits autophagy in cervical cancer cells; promotes xenograft tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circ_0000285 sponges miR197-3p to regulate ELK1 |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); autophagy (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0000285 was associated with tumor size, differentiation, and International Federation of Gynecology and Obstetrics stage |
| Description: |
hsa_circ_0000285 (circ_0000285), derived from HIPK3, is up-regulated in cervical cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes cervical cancer progression by acting as a ceRNA that sponges miR197-3p, thereby relieving repression of ELK1 and enhancing malignant phenotypes; its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.7981 |