| Expression pattern: |
UP |
| Associated gene: |
TJP1 |
| Associated microRNA: |
miR-4720-3p |
| Biological function: |
promotes proliferation and motility (invasion) of gastric cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
acts as a miRNA sponge for miR-4720-3p to regulate TJP1 via a circSMC3/miR-4720-3p/TJP1 axis |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
High circSMC3 expression levels are associated with shorter overall survival in gastric cancer patients. |
| Description: |
circSMC3 is up-regulated in gastric cancer tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-4720-3p, relieving repression of TJP1, thereby promoting gastric cancer cell proliferation and motility and enhancing xenograft tumor growth; high circSMC3 predicts shorter overall survival. |
| Confidence score: |
0.8558 |