| Expression pattern: |
DN |
| Associated gene: |
SHPRH, Ago2 |
| Associated microRNA: |
miR-127-5p, miR-612, miR-4688 |
| Biological function: |
inhibits proliferation and migration; inhibits tumorigenesis; inhibits EMT |
| Molecular mechanism: |
ceRNA mechanism: circ-0001649 sponges miR-127-5p/miR-612/miR-4688 to activate SHPRH |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); EMT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
low expression of circ-0001649 in the tumor tissues was associated with grade of differentiation and tumor satellite |
| Description: |
hsa_circ_0001649 (circ-0001649), derived from the SHPRH locus, is down-regulated in hepatocellular carcinoma tissues and cell lines. It suppresses HCC proliferation, migration and EMT by acting as a ceRNA that sponges miR-127-5p/miR-612/miR-4688, thereby increasing SHPRH expression and inhibiting tumor growth in a xenograft model. |
| Confidence score: |
0.8591 |