| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, N-cadherin, vimentin |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC cell proliferation, migration, invasion and EMT |
| Molecular mechanism: |
circ-SIRT1 binds/recruits EIF4A3 and prevents EIF4A3 recruitment to N-cadherin and vimentin mRNAs, thereby regulating EMT-related protein expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Colony Formation Assay; CCK8; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot |
| Clinical significance: |
circ-SIRT1 expression was positively correlated with the depth of tumor invasion |
| Description: |
circ-SIRT1 is up-regulated in colorectal cancer tissues and cell lines and correlates with deeper tumor invasion. Functionally, circ-SIRT1 promotes CRC cell proliferation, migration/invasion and EMT by binding EIF4A3 and reducing EIF4A3 recruitment to N-cadherin and vimentin mRNAs, thereby increasing EMT marker expression. |
| Confidence score: |
0.6825 |