| Expression pattern: |
DN |
| Associated gene: |
Bcl-2, Cyclin D1, Vimentin, E-cadherin |
| Associated microRNA: |
hsa-miR-1200, hsa-miR-492, hsa-miR-665 |
| Biological function: |
hsa_circ_0036988 suppresses OSCC cell proliferation, migration, invasion and EMT-related malignant behavior; low expression is associated with lymph node metastasis. |
| Molecular mechanism: |
hsa_circ_0036988 regulates the EMT process and alters EMT-related protein expression; bioinformatics predicted potential miRNA targets hsa-miR-1200, hsa-miR-492 and hsa-miR-665, but the detailed regulatory mechanism requires further experimental study. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; ROC Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
Low hsa_circ_0036988 expression was correlated with lymph node metastasis, and ROC analysis suggested diagnostic value as an OSCC indicator. |
| Description: |
This study identifies hsa_circ_0036988 as a down-regulated circRNA in OSCC tissues and cell lines. Functional experiments indicate that hsa_circ_0036988 acts as a tumor suppressor by inhibiting proliferation, migration, invasion and EMT-related changes, while low expression correlates with lymph node metastasis and has potential diagnostic value. |
| Confidence score: |
0.6758 |