circRNA basic information
circBase ID: hsa_circ_0000229
Name: hsa_circ_ZEB1
Synonym: circ-ZEB1
Host Gene: ZEB1
Genomic location(hg19): chr10:31661946-31709678:+
Genomic location(hg38): chr10:31373017-31420749:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

colorectal cancer tissue; adjacent normal mucosal tissue; xenograft tumor tissues; liver metastatic tumors; spleen tumor tissues
Cell lines:

SW480; RKO
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Ago2, BMI1
Associated microRNA: miR-200c-5p / miR-200c
Biological function: Promotes CRC progression, proliferation, colony formation, migration, invasion, tumorigenesis, distant metastasis, EMT, and chemoresistance; circ-ZEB1 knockdown induces G0/G1 arrest and increases chemosensitivity to oxaliplatin and 5-FU.
Molecular mechanism: Acts as a miRNA sponge for miR-200c-5p/miR-200c; via miR-200c regulates its target BMI1, affecting EMT and chemotherapeutic sensitivity; supported by co-localization, dual-luciferase, and Ago2-RIP.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; Sanger Sequencing; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

Potential therapeutic target for CRC treatment.
Description:

circ-ZEB1 (hsa_circ_0000229), derived from ZEB1, is up-regulated in colorectal cancer tissues and promotes malignant phenotypes including proliferation, migration/invasion, tumorigenesis/metastasis, EMT, and resistance to oxaliplatin and 5-FU. Mechanistically, it functions as a ceRNA by sponging miR-200c-5p/miR-200c and thereby relieving repression of BMI1; knockdown phenotypes are rescued by miR-200c inhibition.
Confidence score:

0.7953
Other information
Title:

circ-ZEB1 regulates epithelial-mesenchymal transition and chemotherapy resistance of colorectal cancer through acting on miR-200c-5p.

Journal: Translational oncology
Published: 2023
PubMed ID: 36542990
Study type:

combined biological and clinical study
Data availability: doi:10.1016/j.tranon.2022.101604
Code availability: -