| Expression pattern: |
UP |
| Associated gene: |
YAP1 |
| Associated microRNA: |
miR-646 |
| Biological function: |
promotes high glucose-induced HRPE cell apoptosis, inflammation and ROS generation; knockdown attenuates HG-induced HRPE cell dysfunction |
| Molecular mechanism: |
circ_0000615 acts as a miR-646 sponge to regulate YAP1 expression via the circ_0000615/miR-646/YAP1 axis |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); ceRNA regulation (promotes); Hippo/YAP (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
potential candidate for DR treatment; antagonist of circ_0000615 might be a therapeutic target for DR |
| Description: |
circ_0000615 is up-regulated in high glucose-induced ARPE-19 cells, an in vitro DR cell model. It promotes HRPE cell dysfunction, including apoptosis, inflammation and ROS generation, by sponging miR-646 and thereby regulating YAP1 expression. The study suggests circ_0000615 as a potential therapeutic target for diabetic retinopathy. |
| Confidence score: |
0.7822 |