| Expression pattern: |
UP |
| Associated gene: |
CSE1L |
| Associated microRNA: |
miR-4736 |
| Biological function: |
promotes CRC cell proliferation and metastasis |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge to sequester miR-4736, thereby modulating downstream CSE1L signaling |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0060745 levels significantly correlated with shorter survival time and an ROC AUC value of 0.8442 indicated that circ_0060745 may be a biomarker of CRC. |
| Description: |
circ_0060745 is up-regulated in colorectal cancer and mainly localizes to the cytoplasm. It promotes CRC cell proliferation, migration/invasion and metastasis by sponging miR-4736, thereby relieving repression of the downstream target CSE1L (circ_0060745/miR-4736/CSE1L axis). High circ_0060745 expression is associated with shorter survival time and shows diagnostic potential by ROC analysis. |
| Confidence score: |
0.859 |