circRNA basic information
circBase ID: hsa_circ_0090956
Name: hsa_circ_KIF4A
Synonym: -
Host Gene: KIF4A
Genomic location(hg19): chrX:69606467-69607147:+
Genomic location(hg38): chrX:70386617-70387297:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma samples; adjacent non-tumor tissues; tumor tissues
Cell lines:

LN229; A172; SHG44; U251; NHAs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Wnt5a, beta-catenin, GSK-3beta, p-beta-catenin, c-Myc, cyclin D1, Bcl2, Bax
Associated microRNA: miR-139-3p
Biological function: promotes glioma cell proliferation, migration and invasion; inhibits apoptosis; promotes GICs sphere formation/self-renewal; promotes tumorigenesis in vivo
Molecular mechanism: ceRNA sponging of miR-139-3p to upregulate Wnt5a and activate Wnt/beta-catenin signaling
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); stemness (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model
Clinical significance:

circKIF4A may be a potential target for clinical treatment of glioma.
Description:

circKIF4A is up-regulated in glioma tissues and cell lines and promotes malignant phenotypes (proliferation, migration/invasion, stem-like sphere formation) while inhibiting apoptosis. Mechanistically, it sponges miR-139-3p to upregulate Wnt5a and activate Wnt/beta-catenin signaling, enhancing tumor growth in xenograft models.
Confidence score:

0.6917
Other information
Title:

circKIF4A promotes tumorogenesis of glioma by targeting miR-139-3p to activate Wnt5a signaling.

Journal: Molecular medicine (Cambridge, Mass.)
Published: 2020
PubMed ID: 32268875
Study type:

combined biological and clinical study
Data availability: All data generated or analysed during this study are included in this published article [and its supplementary information files].
Code availability: -