| Expression pattern: |
UP |
| Associated gene: |
VEGFA, GLUT1 |
| Associated microRNA: |
miR-150-5p |
| Biological function: |
Promotes high glucose-induced retinal microvascular endothelial cell injury, apoptosis, tube formation and oxidative stress, while inhibiting cell viability. |
| Molecular mechanism: |
CircZNF609 acts through a sponge/ceRNA mechanism to inhibit miR-150-5p, thereby modulating downstream VEGFA and GLUT1 protein expression and aggravating high glucose-induced hRMEC dysfunction. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); angiogenesis (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Tube Formation Assay; FISH / smFISH; Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
CircZNF609/miR-150-5p axis may be a potential therapeutic target for diabetic retinopathy. |
| Description: |
CircZNF609 is upregulated in peripheral blood from diabetic retinopathy patients and in high glucose-stimulated hRMECs. It aggravates high glucose-induced endothelial cell injury by sponging and suppressing miR-150-5p, leading to reduced viability and increased apoptosis, tube formation, oxidative stress, and VEGFA/GLUT1 protein expression. The CircZNF609/miR-150-5p axis is proposed as a potential therapeutic target for diabetic retinopathy. |
| Confidence score: |
0.6956 |