| Expression pattern: |
UP |
| Associated gene: |
WNT3A, WNT5A, AGO2 |
| Associated microRNA: |
miR-1278 |
| Biological function: |
promotes NSCLC cell proliferation/growth, migration and metastasis; knockdown inhibits tumor growth and lung colonization |
| Molecular mechanism: |
ceRNA mechanism: circ0101675 sponges miR-1278, relieving repression of WNT3A and WNT5A |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); Wnt/beta-catenin (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circ0101675 can be used as a new and useful biomarker for monitoring and treating NSCLC |
| Description: |
circ0101675 is up-regulated in NSCLC tissues and cell lines and promotes malignant phenotypes (proliferation, migration, metastasis). Mechanistically, it acts as a ceRNA to sponge miR-1278, leading to increased WNT3A and WNT5A expression, supporting NSCLC progression. |
| Confidence score: |
0.8442 |