| Expression pattern: |
DN |
| Associated gene: |
beta-catenin, DKK3, AGO2 |
| Associated microRNA: |
miR-1275 |
| Biological function: |
Suppresses LUAD cell proliferation, migration, invasion, tumor growth and metastasis; promotes beta-catenin degradation. |
| Molecular mechanism: |
Direct binding/sequestration of beta-catenin to facilitate its degradation; ceRNA sponging of miR-1275 to relieve suppression of DKK3, inhibiting canonical Wnt/beta-catenin signaling. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; EdU Staining; CCK8; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; ELISA |
| Clinical significance: |
Low circRNF10 expression is associated with advanced tumor stage, larger tumor size, metastasis, and shorter PFS and OS; circRNF10 is an independent protective prognostic factor. |
| Description: |
circRNF10 is down-regulated in driver gene-negative LUAD and acts as a tumor suppressor. It inhibits Wnt/beta-catenin signaling by directly binding beta-catenin to promote its degradation and by sponging miR-1275 to relieve repression of DKK3, thereby reducing proliferation, migration, invasion, and metastasis and associating with better patient survival. |
| Confidence score: |
0.8901 |