| Expression pattern: |
UP |
| Associated gene: |
ATG7, ULK1, AGO2 |
| Associated microRNA: |
miR-1275 |
| Biological function: |
Promotes autophagic flux and promotes proliferation, thereby promoting breast cancer progression in vitro and in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1275 to upregulate ATG7 and ULK1, enhancing autophagic flux. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Cohort Study; Survival Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Microarray; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; ddPCR |
| Clinical significance: |
Increased circCDYL in tumor tissues and serum is associated with higher tumor burden, shorter survival and poorer clinical response to therapy; high serum circCDYL predicts poorer overall survival in MBC. |
| Description: |
circCDYL (hsa_circ_0008285) is up-regulated in breast cancer tissues and serum, and high expression is associated with worse patient outcomes. It promotes breast cancer progression by acting as a miR-1275 sponge to upregulate ATG7 and ULK1, enhancing autophagic flux and proliferation. |
| Confidence score: |
0.8547 |