| Expression pattern: |
UP |
| Associated gene: |
HMGA1 |
| Associated microRNA: |
miR-1294 |
| Biological function: |
CircPLK1 promotes MPM cell proliferation, migration, invasion, stemness and in vivo tumor growth; circPLK1 knockdown suppresses these malignant behaviors. |
| Molecular mechanism: |
CircPLK1 acts as a ceRNA/molecular sponge for miR-1294, thereby relieving miR-1294-mediated repression of HMGA1 and increasing HMGA1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); stemness (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Cohort Study; Survival Analysis; ROC Analysis |
| Clinical significance: |
High circPLK1 expression was associated with low overall survival of MPM patients, and exosomal circPLK1 had diagnostic value for MPM. |
| Description: |
CircPLK1 is up-regulated in MPM tissues, cell lines and serum-derived exosomes, and high expression is associated with poor overall survival and diagnostic potential. Functionally, circPLK1 promotes MPM proliferation, cell-cycle progression, migration, invasion, stemness and xenograft tumor growth. Mechanistically, circPLK1 acts as a cytoplasmic ceRNA that sponges miR-1294 to increase HMGA1 expression. |
| Confidence score: |
0.8583 |