| Expression pattern: |
UP |
| Associated gene: |
JAK/STAT3 signaling pathway, Ago2 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
circ_0038467 knockdown alleviates LPS-induced inflammatory injury by increasing viability, reducing apoptosis, and reducing proinflammatory cytokines (IL-6, IL-8, IL-1beta) in 16HBE cells. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-338-3p and regulates the JAK/STAT3 signaling pathway in LPS-induced inflammatory injury. |
| Biological pathway or process: |
JAK/STAT (promotes); apoptosis (promotes); proliferation (inhibits); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0038467 is up-regulated in LPS-treated human bronchial epithelial (16HBE) cells and promotes inflammatory injury. Its knockdown reduces apoptosis and inflammatory cytokine production and improves cell viability by sponging miR-338-3p and suppressing JAK/STAT3 pathway activation. |
| Confidence score: |
0.7204 |