| Expression pattern: |
UP |
| Associated gene: |
CDC5L, PEAK1 |
| Associated microRNA: |
miR‐542‐3p |
| Biological function: |
promotes proliferation, migration, and invasion and inhibits apoptosis of ovarian cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA/miRNA sponge: circ‐PGAM1 binds miR‐542‐3p with mutual negative feedback, relieving miR‐542‐3p-mediated repression of CDC5L; CDC5L promotes PEAK1 transcription; PEAK1 activates ERK1/2 and JAK2 signaling |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ERK (promotes); JAK/STAT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
- |
| Description: |
Circ-PGAM1 is up-regulated in epithelial ovarian cancer and acts as an oncogenic circRNA. It sponges miR-542-3p (mutual negative feedback), leading to increased CDC5L and downstream PEAK1 transcription, which promotes malignant phenotypes and is associated with activation of ERK1/2 and JAK2 signaling. |
| Confidence score: |
0.7631 |