| Expression pattern: |
UP |
| Associated gene: |
LDHA |
| Associated microRNA: |
miR-409-3p |
| Biological function: |
promotes aerobic glycolysis (Warburg effect) and tumor growth; inhibits apoptosis |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-409-3p, relieving repression of LDHA and enhancing glycolysis. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circATRNL1 expression is correlated with aggressive features and is an independent risk factor for overall survival; high expression indicates unfavorable prognosis. |
| Description: |
circATRNL1 is up-regulated in osteosarcoma tissues and cell lines and is associated with worse overall survival. It mainly localizes to the cytoplasm and promotes aerobic glycolysis and tumor progression by sponging miR-409-3p to increase LDHA expression, also reducing apoptosis; its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.8429 |