circRNA basic information
circBase ID: -
Name: hsa_circ_NSUN2
Synonym: circNSUN2
Host Gene: NSUN2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

-

Cell lines:

SW480; HT29

In vivo animal model:

-

circRNA-disease information
Expression pattern:
DN
Associated gene: STAT3
Associated microRNA: miR-296-5p
Biological function: circNSUN2 promotes malignant development of CRC cells; its overexpression offsets Aloperine-mediated inhibition of proliferation and promotion of apoptosis, while circNSUN2 knockdown inhibits proliferation and promotes apoptosis.
Molecular mechanism: circNSUN2 acts as a miR-296-5p sponge to regulate STAT3; Aloperine suppresses circNSUN2, thereby increasing miR-296-5p and reducing STAT3 activity, leading to reduced proliferation and increased apoptosis.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot

Clinical significance:

-

Description:

In CRC cell lines, Aloperine suppresses circNSUN2, and circNSUN2 promotes malignant phenotypes by sponging miR-296-5p to support STAT3 signaling. Overexpression of circNSUN2 partially rescues Aloperine-induced inhibition of proliferation and promotion of apoptosis, confirming a circNSUN2/miR-296-5p/STAT3 regulatory axis.

Confidence score:

0.6536

Other information
Title:

Aloperine Inhibits Proliferation and Promotes Apoptosis in Colorectal Cancer Cells by Regulating the circNSUN2/miR-296-5p/STAT3 Pathway.

Journal: Drug design, development and therapy
Published: 2021
PubMed ID: 33664565
Study type:

biological research

Data availability: Starbase (http://starbase.sysu.edu.cn/index.php)
Code availability: -