| Expression pattern: |
UP |
| Associated gene: |
ATG7, AGO2 |
| Associated microRNA: |
miR-3657 |
| Biological function: |
Promotes apatinib-induced autophagy and reduces apatinib sensitivity (knockdown sensitizes GC cells to apatinib via autophagy inhibition and promotes apoptosis). |
| Molecular mechanism: |
Acts as a ceRNA/endogenous sponge for miR-3657 to relieve repression of ATG7, thereby promoting autophagy and affecting apatinib sensitivity. |
| Biological pathway or process: |
autophagy (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; TEM; Confocal Microscopy; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
Specific blockage of circRACGAP1 may be a potential therapeutic strategy to reduce the toxicities of apatinib and enhance its therapeutic effect in human GC. |
| Description: |
In gastric cancer under apatinib treatment, circRACGAP1 is induced and functions as a ceRNA sponge for miR-3657, thereby increasing ATG7 and promoting autophagy. Silencing circRACGAP1 suppresses autophagy and increases apoptosis, sensitizing GC cells and xenografts to apatinib. |
| Confidence score: |
0.8572 |