| Expression pattern: |
UP |
| Associated gene: |
Beclin1, RUNX1, ATG13 |
| Associated microRNA: |
miR-199a-5p / miR-199a |
| Biological function: |
promotes autophagy flux, proliferation, invasion and metastasis in EOC |
| Molecular mechanism: |
ceRNA sponging miR-199a-5p to de-repress Beclin1 and RUNX1; RUNX1 transcriptionally elevates circMUC16; circMUC16 directly binds ATG13 to promote its expression |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); ChIP / ChIP-seq; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circMUC16 expression was linked to EOC stage and grade; potential target for EOC diagnosis and therapy |
| Description: |
circMUC16 (hsa_circ_0049116), derived from MUC16, is up-regulated in EOC tissues and patient serum. It promotes EOC autophagy flux and malignant phenotypes (proliferation, invasion/metastasis) by sponging miR-199a-5p to de-repress Beclin1 and RUNX1, forms a RUNX1-circMUC16 positive feedback loop, and also directly binds ATG13 to enhance its expression. |
| Confidence score: |
0.8023 |