circRNA basic information
circBase ID: hsa_circ_0001947
Name: hsa_circ_AFF2
Synonym: circ-AFF2
Host Gene: AFF2
Genomic location(hg19): chrX:147743428-147744289:+
Genomic location(hg38): chrX:148661907-148662768:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008383
MONDO name: rheumatoid arthritis
Disease details: rheumatoid arthritis
Disease DO ID:
 7148
Disease MeSH ID:
 D001172
Disease NCIt ID:
 C2884
Disease ICD11 ID:
 576319925
Disease OMIM ID:
 180300
Species: Human
Species details: Homo sapiens
Tissue specimen:

synovial tissues
Cell lines:

RAFLSs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CNP
Associated microRNA: miR-650
Biological function: promotes proliferation, inflammatory response, migration, invasion, and EMT; inhibits apoptosis in RAFLSs
Molecular mechanism: circ-AFF2 sponges miR-650 to upregulate CNP (ceRNA mechanism)
Biological pathway or process:

proliferation (promotes); inflammation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; ELISA; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down
Clinical significance:

-
Description:

circ-AFF2 (hsa_circ_0001947), derived from AFF2, is up-regulated in RA synovial tissues and primary RAFLSs and mainly localizes to the cytoplasm. Functionally, circ-AFF2 promotes RAFLS proliferation, inflammatory response, migration/invasion and EMT while suppressing apoptosis, by acting as a sponge for miR-650 to de-repress CNP (circ-AFF2/miR-650/CNP axis).
Confidence score:

0.7519
Other information
Title:

Circ-AFF2/miR-650/CNP axis promotes proliferation, inflammatory response, migration, and invasion of rheumatoid arthritis synovial fibroblasts.

Journal: Journal of orthopaedic surgery and research
Published: 2021
PubMed ID: 33653372
Study type:

combined biological and clinical study
Data availability: Not applicable
Code availability: -