| Expression pattern: |
UN |
| Associated gene: |
SIRT7 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
Promotes melanoma cell proliferation, migration and invasion; attenuates apoptosis; suppresses DNA damage; promotes tumor growth in vivo. |
| Molecular mechanism: |
circZNF609 sponges miR-138-5p to regulate SIRT7, thereby repressing DNA damage and promoting malignant progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
In melanoma A375 and SK-MEL-28 cells, circZNF609 promotes proliferation, migration and invasion and inhibits apoptosis while repressing DNA damage. Mechanistically it functions as a ceRNA by sponging miR-138-5p to maintain SIRT7 expression, and circZNF609 knockdown suppresses tumor growth in a nude mouse xenograft model. |
| Confidence score: |
0.5637 |