| Expression pattern: |
UP |
| Associated gene: |
MMP9, AGO2 |
| Associated microRNA: |
miR-194-3p |
| Biological function: |
promotes cell proliferation, colony formation, migration, invasion, tumor growth and pulmonary metastasis in hepatocellular carcinoma |
| Molecular mechanism: |
circUBAP2 acts as a ceRNA to sponge miR-194-3p and thereby upregulates MMP9 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis |
| Clinical significance: |
high circUBAP2 expression is associated with increased tumor size and high tumor recurrence rate and predicts poorer overall survival and recurrence-free survival in HCC patients |
| Description: |
circUBAP2 is up-regulated in hepatocellular carcinoma and promotes proliferation, migration/invasion, tumor growth and lung metastasis. It mainly functions as a cytoplasmic ceRNA by sponging miR-194-3p, thereby relieving repression of MMP9. High circUBAP2 is associated with worse OS/RFS and may serve as a prognostic biomarker and therapeutic target in HCC. |
| Confidence score: |
0.8892 |