| Expression pattern: |
DN |
| Associated gene: |
NDRG1 |
| Associated microRNA: |
miR-223-3p |
| Biological function: |
inhibits proliferation, migration and invasion; induces apoptosis; suppresses xenograft tumor growth |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0003159 sponges miR-223-3p to upregulate NDRG1 |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; H&E Staining; Survival Analysis |
| Clinical significance: |
Low expression of hsa_circ_0003159 was associated with lower overall survival and disease-free survival. |
| Description: |
In gastric cancer, hsa_circ_0003159 is down-regulated and acts as a tumor suppressor by inhibiting proliferation/migration/invasion and promoting apoptosis. Mechanistically, it functions as a ceRNA that sponges miR-223-3p to increase NDRG1 expression, thereby suppressing tumor growth in vitro and in a xenograft model. Low hsa_circ_0003159 expression correlates with worse OS and DFS. |
| Confidence score: |
0.7701 |