| Expression pattern: |
UP |
| Associated gene: |
SKA2 |
| Associated microRNA: |
miR-140-3p |
| Biological function: |
promotes breast cancer cell proliferation, migration, invasion, and glycolysis; supports xenograft tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circ_0008039 sponges miR-140-3p to positively regulate SKA2, affecting HK2 and glycolysis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High expression of circ_0008039 was significantly associated with lymph node metastasis and tumor stage of breast cancer patients. |
| Description: |
circ_0008039 is up-regulated in breast cancer (TNBC) tissues and cells and correlates with advanced stage and lymph node metastasis. Functionally, circ_0008039 promotes proliferation, migration/invasion, and glycolysis. Mechanistically, it acts as a ceRNA by sponging miR-140-3p to increase SKA2 expression, thereby supporting tumor growth in xenografts. |
| Confidence score: |
0.7795 |