| Expression pattern: |
UP |
| Associated gene: |
Snail2, YAP1 |
| Associated microRNA: |
miR-506 |
| Biological function: |
promotes cell proliferation and restrains cell apoptosis (promotes HCC cell viability) |
| Molecular mechanism: |
circPCNX acts as a miR-506 sponge, relieving miR-506-mediated repression of Snail2 and YAP1. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circPCNX levels are associated with larger tumor size and higher TNM stage, and predict lower disease-free survival and overall survival in HCC patients. |
| Description: |
circPCNX is up-regulated in HCC tissues and promotes HCC cell viability in vitro by acting as a miR-506 sponge, thereby increasing expression of miR-506 target oncogenes (e.g., Snail2 and YAP1). High circPCNX expression is associated with worse DFS and OS in HCC patients. |
| Confidence score: |
0.8162 |