| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha, HK2 |
| Associated microRNA: |
miR-487a |
| Biological function: |
promotes proliferation, inhibits apoptosis, and promotes Warburg effect/glycolysis in breast cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circRNF20 sponges miR-487a to relieve repression of HIF-1alpha; HIF-1alpha binds HK2 promoter to promote HK2 transcription, enhancing glycolysis (Warburg effect) |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes); HIF-1 signaling (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; ROC Analysis |
| Clinical significance: |
Increased circRNF20 level predicted the poor clinical outcome in BC specimens; higher circRNF20 expression indicated the lower survival rate of BC patients |
| Description: |
circRNF20 (hsa_circ_0087784), derived from RNF20, is up-regulated in breast cancer and promotes tumor cell proliferation and glycolytic Warburg effect while inhibiting apoptosis. It functions as a ceRNA by sponging miR-487a to increase HIF-1alpha, which transcriptionally activates HK2, thereby enhancing glycolysis and tumor growth; high circRNF20 predicts poor prognosis. |
| Confidence score: |
0.8806 |