| Expression pattern: |
UP |
| Associated gene: |
CDC25B |
| Associated microRNA: |
miR-1200 |
| Biological function: |
Promotes BCa cell proliferation, colony formation, cell cycle progression, migration, tumor growth, and cisplatin chemoresistance. |
| Molecular mechanism: |
CircZNF609 acts as a miR-1200 sponge and relieves miR-1200-mediated inhibition of CDC25B, thereby activating the circZNF609/miR-1200/CDC25B axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); cell cycle (promotes); chemoresistance (promotes); drug resistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
Increased circZNF609 expression is associated with higher histological grade and worse overall survival, and it has potential as a diagnostic biomarker and therapeutic target in BCa. |
| Description: |
CircZNF609 is up-regulated in bladder cancer tissues and cell lines and high expression is associated with worse overall survival. It promotes BCa proliferation, migration, cell cycle progression, tumor growth, and cisplatin resistance by sponging miR-1200 to increase CDC25B expression. |
| Confidence score: |
0.8814 |