| Expression pattern: |
DS |
| Associated gene: |
Ago2, MEF2A, Tie2, IGF2 |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
cZNF609 silencing decreased retinal vessel loss, suppressed pathological angiogenesis, increased endothelial cell migration and tube formation, and protected endothelial cells against oxidative stress/hypoxia-induced apoptosis. |
| Molecular mechanism: |
Acts as a miRNA sponge: cZNF609 binds miR-615-5p (Ago2-associated), reducing miR-615-5p activity and increasing MEF2A expression; MEF2A mediates endothelial phenotypes. |
| Biological pathway or process: |
angiogenesis (inhibits); migration (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; ELISA |
| Clinical significance: |
cZNF609 expression was higher in fibrovascular membranes and plasma of diabetic patients, while circulating cZNF609 was down-regulated in CAD or hypertension patients compared with healthy volunteers. |
| Description: |
cZNF609 is dysregulated during vascular dysfunction and modulates endothelial stress responses and angiogenic behavior. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-615-5p to de-repress MEF2A, affecting migration, tube formation, and apoptosis, and its silencing reduces retinal vessel loss and pathological angiogenesis in mouse retinopathy models. |
| Confidence score: |
0.8437 |