circRNA basic information
circBase ID: hsa_circ_0000140
Name: hsa_circ_KIAA0907
Synonym: -
Host Gene: KIAA0907
Genomic location(hg19): chr1:155891165-155895634:-
Genomic location(hg38): chr1:155921374-155925843:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004958
MONDO name: oral cavity squamous cell carcinoma
Disease details: Oral squamous cell carcinoma
Disease DO ID:
 0050866
Disease MeSH ID:
 -
Disease NCIt ID:
 C4833
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OSCC tissues; adjacent normal tissues; lymph node metastasis samples; xenograft tumor tissues; lung tissues
Cell lines:

HOK; Cal-27; SCC-25; SCC-9; HSC-3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: LATS2, AGO2, YAP1, TAZ
Associated microRNA: miR-31
Biological function: Suppresses OSCC cell proliferation, migration and invasion, promotes apoptosis, and inhibits EMT, thereby repressing tumor growth and lung metastasis.
Molecular mechanism: Acts as a miRNA sponge for miR-31, thereby up-regulating LATS2 and suppressing Hippo pathway output (reduced YAP1 nuclear translocation), leading to inhibition of EMT and metastasis.
Biological pathway or process:

Hippo/YAP (inhibits); EMT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circ_0000140 levels correlate with higher lymph node metastasis, advanced TNM stage, and poor overall survival in OSCC patients.
Description:

circ_0000140 (from KIAA0907) is down-regulated in OSCC and functions as a tumor suppressor. It sponges miR-31 to increase LATS2, inhibit Hippo/YAP signaling output and EMT, thereby reducing proliferation, invasion/migration and lung metastasis, and higher circ_0000140 associates with better survival.
Confidence score:

0.8814
Other information
Title:

circRNA_0000140 suppresses oral squamous cell carcinoma growth and metastasis by targeting miR-31 to inhibit Hippo signaling pathway.

Journal: Cell death & disease
Published: 2020
PubMed ID: 32041942
Study type:

combined biological and clinical study
Data availability: Supplementary Information accompanies this paper at 10.1038/s41419-020-2273-y.
Code availability: -