circRNA basic information
circBase ID: hsa_circ_0003204
Name: hsa_circ_USP36
Synonym: -
Host Gene: USP36
Genomic location(hg19): chr17:76798405-76800060:-
Genomic location(hg38): chr17:78802323-78803978:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HCtAECs; THP-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TRPC6, Ago2
Associated microRNA: miR-188-3p
Biological function: Promotes ox-LDL-induced cell injury: enhances apoptosis in HCtAECs and promotes inflammatory response and oxidative stress in THP-1 cells.
Molecular mechanism: Acts as a ceRNA to sponge miR-188-3p and thereby upregulate TRPC6 (validated by dual-luciferase reporter and Ago2-RIP).
Biological pathway or process:

apoptosis (promotes); inflammation (promotes); other pathway/process (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

In ox-LDL-treated HCtAECs and THP-1 cells (in vitro carotid atherosclerosis model), circ_0003204 is up-regulated and promotes cell injury. Mechanistically, circ_0003204 acts as a ceRNA that sponges miR-188-3p to increase TRPC6, thereby enhancing endothelial apoptosis and promoting inflammatory response and oxidative stress; circ_0003204 knockdown is protective.
Confidence score:

0.627
Other information
Title:

Hsa_circ_0003204 Knockdown Weakens Ox-LDL-Induced Cell Injury by Regulating miR-188-3p/TRPC6 Axis in Human Carotid Artery Endothelial Cells and THP-1 Cells.

Journal: Frontiers in cardiovascular medicine
Published: 2021
PubMed ID: 34912856
Study type:

biological research
Data availability: https://www.frontiersin.org/articles/10.3389/fcvm.2021.731890/full#supplementary-material
Code availability: -