circRNA basic information
circBase ID: hsa_circ_0122319
Name: hsa_circ_PLOD2
Synonym: circPLOD2
Host Gene: PLOD2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021063
MONDO name: malignant colon neoplasm
Disease details: colon cancer
Disease DO ID:
 219
Disease MeSH ID:
 -
Disease NCIt ID:
 C9242
Disease ICD11 ID:
 1265576634
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

colon cancer tissues; adjacent tumor-free tissues; tumor samples
Cell lines:

SW620; HCT-116; HT-29; LoVo; RCM-1; HEK293; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SIX1, LDHA, AGO2
Associated microRNA: miR-513a-5p
Biological function: Promotes colon cancer cell proliferation, migration, invasion, tumorigenicity, and Warburg effect; circPLOD2 knockdown inhibits these malignant phenotypes.
Molecular mechanism: circPLOD2 acts as a miR-513a-5p sponge to activate SIX1; SIX1 enhances LDHA transcription and regulates glycolysis/Warburg effect in colon cancer cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circPLOD2 expression is associated with shorter overall survival and may act as a biomarker for colon cancer prognosis, diagnosis, and treatment.
Description:

circPLOD2 is upregulated in colon cancer tissues and cell lines and high expression is associated with poor overall survival. Functionally, circPLOD2 promotes proliferation, migration, invasion, tumor growth, and glycolytic/Warburg metabolism. Mechanistically, it sponges miR-513a-5p to activate SIX1, which enhances LDHA transcription and supports colon cancer progression.
Confidence score:

0.8814
Other information
Title:

circRNA PLOD2 promotes tumorigenesis and Warburg effect in colon cancer by the miR-513a-5p/SIX1/LDHA axis.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 36071678
Study type:

combined biological and clinical study
Data availability: TCGA; circInteractome; miRDB; miRWalk; TargetScan
Code availability: -