circRNA basic information
circBase ID: hsa_circ_0021506
Name: hsa_circ_ANO5
Synonym: circular RNA ANO5
Host Gene: ANO5
Genomic location(hg19): chr11:22225349-22249132:+
Genomic location(hg38): chr11:22203803-22227586:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tumor tissues; normal tissues; generated tumors
Cell lines:

GES-1; AGS; HGC-27
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: LIFR
Associated microRNA: miR-21-5p
Biological function: circ_ANO5 acts as a tumor-suppressive circRNA in gastric cancer; its upregulation by lidocaine inhibits GC cell proliferation, clonogenicity, migration, invasion and tumor growth, and promotes apoptosis.
Molecular mechanism: circ_ANO5 functions through a ceRNA mechanism by sponging miR-21-5p, thereby relieving miR-21-5p-mediated suppression of LIFR and mediating the anti-GC effects of lidocaine.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other); other pathway/process (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Actinomycin D / DRB Stability Assay; Transfection; Colony Formation Assay; MTT; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_ANO5 is downregulated in gastric cancer cells and GC tumor tissues from GSE83521, while lidocaine increases its expression. In this study, circ_ANO5 mediates lidocaine-induced suppression of GC proliferation, migration, invasion, EMT-related changes and xenograft tumor growth, while promoting apoptosis. Mechanistically, circ_ANO5 sponges miR-21-5p to regulate LIFR expression, forming a circ_ANO5/miR-21-5p/LIFR axis.
Confidence score:

0.6742
Other information
Title:

Lidocaine Suppresses Gastric Cancer Development Through Circ_ANO5/miR-21-5p/LIFR Axis.

Journal: Digestive diseases and sciences
Published: 2022
PubMed ID: 34050852
Study type:

combined biological and bioinformatics study
Data availability: GSE83521
Code availability: -