circRNA basic information
circBase ID: hsa_circ_0005273
Name: hsa_circ_PTK2
Synonym: circPTK2
Host Gene: PTK2
Genomic location(hg19): chr8:141710989-141716304:-
Genomic location(hg38): chr8:140700890-140706205:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tumor tissue; adjacent non-tumor tissue; serum; peripheral blood; tissue microarray (TMA)
Cell lines:

HCT15; SW620; SW480; LOVO; 293 T
In vivo animal model:

patient-derived xenograft; cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: vimentin, MMP2/9, CXCR4, E-cadherin
Associated microRNA: -
Biological function: Promotes CRC tumor growth and metastasis; promotes EMT; promotes proliferation, migration and invasion; affects apoptosis in a cell-context dependent manner.
Molecular mechanism: circPTK2 physically binds vimentin and targets its phosphorylation sites (Ser38, Ser55, Ser82), promoting EMT and metastatic phenotypes.
Biological pathway or process:

EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (other)
Detected method:
Q
H
M
Validation methods:

Microarray; RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining
Clinical significance:

High tumoral circPTK2 is associated with shorter overall survival; circulating (serum) circPTK2 is elevated in CRC and indicative of CRC with node or distant metastases.
Description:

circPTK2 (hsa_circ_0005273), derived from PTK2, is up-regulated in CRC tissues and serum and is associated with metastasis and poorer overall survival. Functionally, circPTK2 promotes aggressive phenotypes and EMT in vitro and in vivo, at least in part by physically binding vimentin (Ser38/Ser55/Ser82) and increasing vimentin and related EMT/metastasis markers.
Confidence score:

0.7876
Other information
Title:

CircPTK2 (hsa_circ_0005273) as a novel therapeutic target for metastatic colorectal cancer.

Journal: Molecular cancer
Published: 2020
PubMed ID: 31973707
Study type:

combined biological and clinical study
Data availability: The data used or analyzed during this study are included in this article and available from the corresponding author upon reasonable request.
Code availability: -