| Expression pattern: |
DN |
| Associated gene: |
TP63, ∆NP63 |
| Associated microRNA: |
miR-762 |
| Biological function: |
inhibits migration, invasion and proliferation of UCB cells; suppresses tumor growth in vivo |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge of miR-762 to increase ∆NP63 (TP63 isoform) expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Bioinformatics Analysis; RNA-seq; RT-qPCR; Clinical Sample Validation; divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circFAM114A2 expression was highly correlated with pathological TNM stage and grade |
| Description: |
circFAM114A2 is down-regulated in urothelial carcinoma of bladder and acts as a tumor suppressor. It sponges miR-762 in the cytoplasm to relieve repression of TP63/∆NP63, thereby inhibiting bladder cancer cell proliferation, migration, and invasion and suppressing xenograft tumor growth. |
| Confidence score: |
0.8635 |