| Expression pattern: |
UP |
| Associated gene: |
SIX2 |
| Associated microRNA: |
miR-335-5p |
| Biological function: |
promotes oxygen consumption, colony formation, migration and invasion; promotes xenograft tumor growth |
| Molecular mechanism: |
ceRNA/miRNA sponge: circ_0007255 sponges miR-335-5p to regulate SIX2 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); oxidative phosphorylation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; ROC Analysis; Clinical Sample Validation |
| Clinical significance: |
ROC curves exhibited apparent separation between BC tissues and the healthy donators, with an AUC of 0.7748 for circ_0007255; might serve as a novel diagnostic and prognostic biomarker |
| Description: |
circ_0007255 is up-regulated in breast cancer serum/tissues/cell lines and mainly localizes to the cytoplasm. It promotes malignant behaviors and xenograft tumor growth by sponging miR-335-5p, thereby increasing SIX2 expression, suggesting diagnostic potential (ROC AUC reported). |
| Confidence score: |
0.8025 |