| Expression pattern: |
DN |
| Associated gene: |
Rac1, Cdc42, cyclin B1, RhoA, p21, p27 |
| Associated microRNA: |
miR-29b |
| Biological function: |
Promotes intestinal epithelial repair after wounding and supports epithelial renewal/proliferation; enhances actin-based protrusions and migration. |
| Molecular mechanism: |
CircHIPK3 directly binds and sequesters miR-29b, inhibiting miR-29b activity and thereby increasing Rac1, Cdc42 and cyclin B1 expression in IECs after wounding. |
| Biological pathway or process: |
migration (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Clinical Sample Validation; FISH / smFISH; Transfection; RNA Pull-Down; Luciferase Reporter Assay; MTT; BrdU; In Vivo Animal Model; Western Blot |
| Clinical significance: |
Intestinal mucosal tissues from patients with active CD and UC and sepsis showed decreased circHIPK3 levels and were associated with severe mucosal injury/erosions, inflammation, delayed healing and gut barrier dysfunction. |
| Description: |
circHIPK3 is reduced in human intestinal mucosa from IBD and sepsis patients, and it promotes intestinal epithelial wound repair and epithelial renewal. Mechanistically, it acts as a miR-29b sponge to relieve miR-29b-mediated repression of targets such as Rac1, Cdc42 and cyclin B1, supporting migration/proliferation during restitution. |
| Confidence score: |
0.7726 |