circRNA basic information
circBase ID: hsa_circ_0061395
Name: hsa_circ_BACH1
Synonym: Circular RNA_0061395
Host Gene: BACH1
Genomic location(hg19): chr21:30698379-30702014:+
Genomic location(hg38): chr21:29326058-29329693:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

liver cancer tissues; normal liver tissues; liver tumor specimens; para-hepatic normal liver tissues; tumor tissues
Cell lines:

THLE-2; Huh-7; MHCC97 H; SNU-387; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SPOCK1, BACH1 mRNA, Ago2
Associated microRNA: miR-1182
Biological function: promotes HCC cell proliferation, migration, invasion, angiogenesis, tumor spheroid formation, EMT and tumor growth; inhibits apoptosis
Molecular mechanism: circ_0061395 acts as a sponge for miR-1182 and positively regulates SPOCK1 expression through the miR-1182/SPOCK1 axis
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); EMT (promotes); stemness (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

potential targeted therapy or cure target for HCC
Description:

circ_0061395 is up-regulated in HCC tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion, angiogenesis, tumor spheroid formation, EMT and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0061395 sponges miR-1182 to increase SPOCK1 expression, forming a circ_0061395/miR-1182/SPOCK1 regulatory axis.
Confidence score:

0.8175
Other information
Title:

Circ_0061395 functions as an oncogenic gene in hepatocellular carcinoma by acting as a miR-1182 sponge.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 35775884
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request.
Code availability: -