| Expression pattern: |
UP |
| Associated gene: |
YTHDC1, IGF2BP2, HMGA2, METTL3, CXCR4 |
| Associated microRNA: |
- |
| Biological function: |
promotes liver metastasis and invasion/migration of CRC cells; enhances EMT |
| Molecular mechanism: |
m6A-dependent nuclear export mediated by YTHDC1; formation of circNSUN2/IGF2BP2/HMGA2 ternary complex to stabilize HMGA2 mRNA |
| Biological pathway or process: |
metastasis (promotes); invasion (promotes); migration (promotes); EMT (promotes); m6A modification (other); mRNA stability (promotes) |
| Detected method: |
Q
B
M
|
| Validation methods: |
Microarray; RNase R Treatment; divergent primers PCR; RT-qPCR; Sanger Sequencing; Northern Blot; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Survival Analysis; In Vivo Animal Model; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); CLIP; MeRIP / MeRIP-seq; RNA-seq; Western Blot; IF (Immunofluorescence); Luciferase Reporter Assay; Transwell Assay |
| Clinical significance: |
frequently upregulated in tumor tissues and serum samples from CRC patients with liver metastasis and predicts poorer patient survival; higher in LM tissues than in primary CRC tissues |
| Description: |
circNSUN2 is up-regulated in CRC with liver metastasis (in tumor tissues and serum) and is associated with poor survival. Its m6A-dependent export (via YTHDC1) increases cytoplasmic circNSUN2, which forms a ternary complex with IGF2BP2 and HMGA2 to stabilize HMGA2 mRNA, promoting invasion/EMT and liver metastasis. |
| Confidence score: |
0.8798 |